| Welcome of participants |
| 8h - 9h30 | Welcome of participants and poster settings |
| 9h30 - 9h35 | Welcome address : C. Beaumont, P. Brissot |
| 9h35 - 10h15 | Opening Conference : "The iron foundations of life" G. Wächtershäuser |
| 10h15 - 12h15 | Plenary Session 1 A : Regulation of iron - dependent gene expression |
| Chair : E. Theil, A. Pietrangelo | |
| 10h15 | Invited lecture : Regulation of siderophore-mediated iron transport : mysteries and certainties S. Leong |
| 11h - 11h30 | Coffee break |
| 11h30 | Regulation of ferritin H transcription by the E1A oncogene : interaction of the ferritin H enhancer, FER-1 with members of the AP1 and SP1 transcription factor families. Tsuji Y., Torti S.V., Torti F.M . |
| 11h45 | Regulation of the expression of the ZmFer1 maize ferritin gene in response to iron. Savino G., Briat J.F., Lobréaux S. |
| 12h00 | Differential display of intestinal mRNAs regulated by intracellular Fe levels in CaCo-2 cells. Arredondo M., Berroteran R., Glass J. |
| 12h30 | Lunch (Salle du Grand Large) |
| 14h - 15h45 | Plenary Session 1 B : HLA-H : gene and protein | |
| Chair : J.Y. LeGall - E.C. Jazwinska. | ||
| 14 h | Invited lecture : Molecular genetics of hemochromatosis W. Thomas |
|
| 14h30 | Immunohistochemistry of HFE, the protein defective in patients with hereditary hemochromatosis, reveals unique pattern of expression in small intestine. Parkkila S., Waheed A., Britton R.S., Feder J.N., Tsuchihashi Z., Schatzman R.C., Bacon B.R., Sly W.S. | |
| 14h45 | Iron regulation of the hemochromatosis gene (HLA-H) in human intestinal CaCo-2 cells. Han O., Fleet J.C., Wood R.J. | |
| 15h00 | Differences in the subcellular localisation of the mutated and non-mutated forms of HLA-H. Banyer J.L., Thomson S.A., Hobson K., Balding K., Powell L.W., Jazwinska E.C., Khanna K., Anderson G.J. | |
| 15h15 | Defective iron homeostasis in b2-microglobulin knockout mice recapitulates hereditary hemochromatosis in man. Santos M., Schilham M.W., Rademakers L.H., Marx J.J.M., de Sousa M., Clevers H. | |
| 15h30 | Structural and evolutionary studies on the hemochromatosis gene. Elshof A.T., Gerhard G., Hughes A., Bahram S., Chorney M. | |
| 15h45 - 16 h15 | Coffee break | |
| 16h15 - 18h00 |
| Proteins of iron metabolism | Disorders of iron metabolism | |
| Structure/ function relationships |
Classification and diagnosis, prevalence of HLA-H mutations |
|
| Chair : P. Arosio, P. Harrison | Chair : P. Brissot, V. Gordeuk. | |
| 16h15 - 16h45 | Invited lecture : Interactions of lactoferrins with their target cells G. Spik |
Invited lecture : Classification and diagnosis P. Adams |
| 16h45 | Does transferrin perform a molecular conformation search ? Crawley J.B., Evans R.W., Grossmann J.G., Hasnain S.S., Strange R.W. | Poster review and round-table discussion : "Prevalence of HLA-H mutations". Posters from the following |
| 17h00 | The assembly of iron-sulfur clusters in proteins : what simple systems can tell about the final stages of biological iron mobilisation ? Moulis J.M. | authors will be discussed : |
| 17h15 | Remarkable ability of horse spleen apoferritin to demetallate haem and to metallate protoporphyrin IX as a function of pH. Crichton R., Soruco J.A., Roland F., Michaux M.A., Gallois B., Précigoux G., Mahy J.P., Mansuy D. | H. Bell, C. Camaschella, E.M. Cardoso, G. Jeffrey, P.A.
Martinez, A.T. Meryweather-Clarke, R. Moirand, P. Nielsen, K.S. Olsson, A. Piperno, G. Porto, M.P. Roth, R. Sham, M. Worwood. |
| 17h30 | Structure and cloning of serum ferritin. Linder M.C., Hazegh-Azam M., Schaffer K.J., Butcher L.,Zhou C.Y.J., Nguyen A., Goode C.A., Nagel G.M., Hallock M. | |
| 17h45 | Study of the receptor for human H-ferritin in K562 cells. Santambrogio P., Altimare D., Nardone E., Levi S., Arosio P. |
| 18h00 | End of Parallel Sessions 1 |
| 9h - 12h30 | Plenary Session 2 : Iron as a damaging co-factor |
| Chair : B. Bacon, R. Ward. | |
| 9h00 | Invited lecture: Oxidant and anti-oxidant regulation of signal transduction L. Packer |
| 9h45 | Induction of ferritin synthesis : molecular analysis of a protective mechanism against oxidative damage in post-ischemic reperfusion. Cairo G., Tacchini L., Recalcati S., Azzimonti B., Bernelli-Zazzera A. |
| 10h00 | Tissue ferritin induces nitric oxide production by hepatic stellate cells : role of ferritin receptor and PKCz in activation cascade. Ramm G.A., Piva T.J., Nair V.G., Garrone B.M., Watters D.J., Crawford D.H.G. |
| 10h15 | Primary cultures of rat hepatocytes are protected from oxidative stress by TNF-a. Hagen K., Zhu C., Melefors O., Hultcrantz R. |
| 10h30 - 11h00 | Coffee break |
| 11h00 - 12h30 | Plenary Session 2 (continued ) : Iron as a damaging (co)factor |
| Chair : D. Conte, J. Glass. | |
| 11h00 | Invited lecture : Iron and neoplasia S. Fargion |
| 11h30 | Sensitization of cultured skin fibroblasts to ultraviolet a (320-400 NM) by excess of iron. Morliè re P., Salmon S., Giordani A., Aubailly M., Risler A., Santus R. |
| 11h45 | Invited lecture : HIV and iron : state of the art J. Boelaert |
| 12h15 | Iron-induced acute tubular necrosis and renal cell carcinoma-alleviation by estrogen and vitamin E. Okada S.E., Shang D., Takahiro K., Deguchi J. |
| 12h30 | Quick Lunch (Salle du Grand Large) |
| 13h30 - 16h00 | Poster viewing (poster No 74 to 220) | 16h00 - 18h00 | Plenary Session 3 : Iron and erythropoiesis |
| Chair : J. Halliday, M. Pippard. | |
| 16h00 | Plenary Conference : "Mechanisms of erythroid-specific gene expression" S. Orkin |
| 17h00 | Regulation of cellular iron metabolism by erythropoietin via activation of iron-regulatory protein-1. Weiss G., Houston T., Kastner S., Johrer K., Zoller H., Brock J.H. |
| 17h15 | Utilization and recognition of extra- and intracellular ferritin iron by developing human erythroid precursors in culture. Vaisman B., Meyron-Holts E.G., Leimberg J.M., Fibach E., Rouault T.A., Konijn A.M. |
| 17h30 | Role of ferritin in the control of the labile iron pool in murine erythroleukemic cells. Picard V., Epsztejn S., Cabantchik Z.I., Beaumont C. |
| 17h45 | Increased mass of transferrin receptors and functional iron deficiency in response to treatment of normal animals with recombinant human erythropoietin. Loo M., Fillet G., Beguin Y. |
| 18h00 | Invited lecture : Tissue-specific regulation of iron metabolism and heme synthesis P. Ponka |
| 9h00 - 10h15 | Plenary Session 4 : Iron in the brain |
| Chair : J. Connor, D. Baxter. | |
| 9h00 | Invited lecture : Iron in the brain, its relation to neurological disorders D. Baxter. |
| 9h45 | Altered ferritin and transferrin receptor distribution around brain lesions in multiple sclerosis. Hulet S.W., Powers S., Connor J.R. |
| 10h00 | Major fractional appearance of non-transferrin-bound 59Fe in rat cerebrospinal fluid following injection of 59Fe-transferrin into peripheral blood. Moos T., Morgan E.H. |
| 10h15 | A major decrease of retinal transferrin expression but not ferritin is correlated with the onset of the disease in a hereditary rat model of retinal degeneration. Yefimova M., Guillonneau X., Jeanny J.C., Courtois Y. |
| 10h30 - 11h00 | Coffee break |
| 11h00 - 12h30 |
| Proteins of iron metabolism | Disorders of iron metabolism | |
| Regulation of gene expression | Porphyria, Thalassemia, Sideroblastic anemia | |
| Chair : E. Leibold, I. Cabantchik. | Chair : M. Worwood. | |
| 11h00 - 11h30 | Invited lecture : The iron-sulfur switch in relation with iron, NO and oxidative stress. M. Hentze |
Invited lecture : Iron and Porphyria J. Kushner |
| 11h30 | Hydrogen peroxide is a signal for the induction of iron regulatory protein when cells are released from GO into the cell cycle. Robalinho R.L., Meneghini R. | Short oral presentation of four posters on HLA-H mutations in PCT
patients, by : |
| 11h45 | Mutation of a possible PKC phosphorylation site in iron regulatory protein 1 eliminates aconitase activity without affecting the RNA binding function. Eisenstein R.S.,Carpenter T.B., Kennedy M.C., Brown N.M., Walden W. | G.H.Elder, R. Raha-Chowdhury, M. Sampietro, M. Santos. |
| 12h00 | Iron regulatory protein-2 represses translation in cells. Menotti E., Kühn L.C. | X-linked sideroblastic anemia due to mutations in the erythroid 5-aminolevulinate synthase gene is commonly manifested in females. Bottomley S.S., Wise P.D., Wasson E.G., Carpenter N.J |
| 12h15 | Iron-dependent degradation of iron regulatory protein 2 (IRP2) is associated with oxidative modification and ubiquitination of the protein. Iwai K., Drake S.K., Wehr N., Klausner R.D., Weissman A.N., Levine R.L., Rouault T.A. | Heteroplasmic point mutations of mitochondrial DNA affecting subunit I of cytochrome-C-oxidase in two patients with acquired idiopathic sideroblastic anemia. Gattermann N., Retzlaff S., Wang Y.L., Aul C., Schneider W. |
| 12h30 | End of the Session and Free time for Lunch |
| 9h00 - 10h30 | Plenary Session 5 : Iron transport through biological membranes |
| Chair : T. Tavill, N. Grace. | |
| 9h00 | Invited lecture : "The iron and copper connection" A. Dancis |
| 9h45 | "Bacterial iron transport" V. Braun |
| 10h30 - 11h00 | Coffee break | 11h00 - 12h30 | Plenary Session 5 (continued ): Iron transport through biological membranes |
| Chair : P. Aisen, G. Anderson. | |
| 11h00 | Apotransferrin and holotransferrin undergo different endocytic cycles in intestinal epithelia (CACO-2) cells. Nunez M.T. |
| 11h15 | Inhibition of the uptake of transferrin-bound iron by transferrin-independent pathway by non-transferrin bound iron. Trinder D., Morgan E.H. |
| 11h30 | The gene defective in microcytic mice encodes a critical intestinal iron transport protein. Fleming M.D., Trenor C.C., Su M.S., Dietrich W.F., Andrews N.C. |
| 11h45 | Isolation of novel duodenum specific genes which demonstrate regulation by iron. McKie A.T., Simpson R.J., Peters T.J., Farzaneh F. |
| 12h00 | Identification and cloning of a putative iron-transporter in K562 cells. Pountney D.J., Grandchamp B., Beaumont C. |
| 12h15 | Expression cloning and characterization of a mammalian proton-coupled divalent cation transporter (DCT1) from rat duodenum. Gunshin H., MacKenzie B., Berger U.V., Gunshin Y., Romero M.F., Boron W.F., Nussberger S., Gollan J.L., Hediger M.A. |
| 12h30 | Quick Lunch (Salle du Grand Large) |
| 13h30 - 14h30 | Poster viewing : ( poster No 221 to 325) |
| 14h30 - 15h00 | Plenary Conference : "Iron-related disorders throughout the world" L. Powell |
| 15h00 - 15h30 | Business meeting : ( Organisation of future iron conferences) |
| 15h30 - 17h00 |
| Proteins of iron metabolism | Disorders of iron metabolism | |
| Siderophore-dependant and -independant iron uptake in pathogenic and non-pathogenic microorganisms. | New insights into iron disorders | |
| Chair : J.F. Briat, R. Hider. | Chair : M. de Sousa, Y. Deugnier. | |
| 15h30 | Biomimetic iron carriers: from structural and functional probes to diagnostic agents. Shanzer A. | Reversal of the CD8+CD28+/CD8+CD28- ratio in peripheral blood of hereditary haemochromatosis patients correlation with HLA-A3 phenotype and severity of iron overload. Arosa F.A., Porto G., Lacerda R., De Sousa M. |
| 15h45 | Study of the iron transport in Pseudomonas aeruginosa ATCC 15692 by the photolabelling technique. Schalk I., Ocaktan A., Rochet L., Bernardini J.J., Hennard C., Kyslik P., Abdallah M.A. | Role of iron on protein kinase C activation : implication on impaired lymphocyte proliferation and immune responses associated with iron deficiency. Kuvibidila S., Baliga B.S. |
| 16h00 | Regulatory factors of the iron transport-pectinolysis connection in erwinia chrysanthemi. Expert D., Franza T., Piquerel P., Sauvage C. | Short oral presentation of four posters on the hereditary
cataract-hyperferritinemia syndrome by : |
| 16h15 | Identification and characterization of yeast genes involved in intracellular iron metabolism and transport. Kaplan J., deSilva D., Li L., Kaplan S.D., Babcock M. | C. Beaumont, M. Cazzola, D. Girelli, S. Levi. |
| 16h30 | In vitro characterisation of the interaction between meningococcal transferrin binding proteins and human-bovine chimeric transferrins using surface plasmon resonance. Boulton I.C., Gorringe A.R., Gorinsky B., Shergill J., Joannou C.L., Patel K.J. | HLA-H mutations in patients with iron overload and normal transferrin saturation. Deugnier Y., Moirand R., Jouanolle A.M., Le Gall J.Y., Brissot P., David V. |
| 16h45 | The host protein independent iron uptake by tritrichomonas foetus. Suchan P., Kulda J., Shrével J., Tachezy J. | Iron overload with normal transferrin saturation : a subset of genetic hemochromatosis ? Fargion S., Sampietro M., Fracanzani A.L., Cairo G., Arosio P., Lupica L., Dimasi V., Corbetta N., Fiorelli G. |
| 17h00 | End of Parallel Sessions 3 |
| Satellite meetings |
| Genetics of Hemochromatosis | Iron Chelators |